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KMID : 0364820210570030174
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Korean Journal of Microbiology
2021 Volume.57 No. 3 p.174 ~ p.182
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Screening and molecular identification of Streptomyces species isolated from high altitude soil of Nepal
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Pandey Bishnu Prasad
Pradhan Suman Prakash
Adhikari Kapil
Shrestha Rajib Kumar
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Abstract
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Streptomyces are widely distributed in soil and known for the production of bioactive secondary metabolites. It has been reported that among microbial-derived antibiotics, two-third are produced by the Streptomyces species alone. Hence, continuous screening of the Streptomyces species is of growing scientific interest. A small Himalayan country like Nepal is in a unique geographical location with a huge biodiversity. However, little is known about microbial diversity. The aim of this study was to isolate and characterize the Streptomyces species from a high-altitude soil sample collected from an altitude of 4,380 meters above sea level. The 16S rRNA sequence analysis revealed that four isolated strains; G-10, G-14, G-18, and S4L belong to the Streptomyces species. On the basis of phylogenetic analysis of 16S rRNA gene sequences of the isolates with the best match in the database revealed that G-18 isolates closely related to Streptomyces albidoflavus strain PAS-12. Moreover, the ranges of radical scavenging activities by crude extract of isolates were observed against DPPH and ABTS. Furthermore, crude extract of isolates revealed the range of antimicrobial activities against the four pathogenic strains namely Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus species, and Bacillus substilis. Moreover, isolated Streptomyces species revealed amylase, cellulase, and L-asparaginase enzyme activities.
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KEYWORD
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Streptomyces, 16S rRNA, antioxidant, anti-microbial, soil
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